thr 77 - An Overview
thr 77 - An Overview
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Antibodies certain to the blocking peptide not bind into the epitope around the goal protein. By evaluating the staining from your blocked antibody versus the antibody by yourself, one can see which staining is precise.
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This guideline outlines further more achievable optimization of mobile and immunoassay parameters to make certain the very best final results are...
This guideline outlines additional doable optimization of mobile and immunoassay parameters to make sure the absolute best success are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized via the antibody.
Concentrate on Information and facts Cyclin E belongs towards the highly conserved cyclin family members, whose members are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation patterns which add to the temporal coordination of each mitotic party. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is degraded as cells development through S section.
Overexpression of the gene is noticed in several tumors, which ends up in chromosome instability, and thus may contribute to tumorigenesis. This protein was located to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial purpose in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two supplemental splice variants had been described but comprehensive nucleotide sequence facts is not nevertheless out there.
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